Xylitol Formation and Key Enzyme Activities in Candida boidinii under Different Oxygen Transfer Rates
نویسندگان
چکیده
Under oxygen transfer rates (OTR), from 10 to 30 mmol. l –1 h , Candida boidinii NRRL Y-17213 exhibited both NADH and NADPH linked D-xylose reductase activities with the former being higher. Xylitol dehydrogenase was mainly NAD dependent. Maximum xylitol production was attained at OTR of 14 mmol. l h . Ethanol, glycerol and ribitol were also produced. A correlation between xylitol accumulation, oxygen availability and key enzyme activities was viewed.
منابع مشابه
Molecular characterization of a gene for aldose reductase ( CbXYL1) from Candida boidinii and its expression in Saccharomyces cerevisiae.
Candida boidinii produces significant amounts of xylitol from xylose, and assays of crude homogenates for aldose (xylose) reductase (XYL1p) have been reported to show relatively high activity with NADH as a cofactor even though XYL1p purified from this yeast does not have such activity. A gene coding for XYL1p from C. boidinii (CbXYL1) was isolated by amplifying the central region using primers...
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